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Welcome to MSEQER

a tool for MSAP-seq data analysis
About program

Several methods of DNA methylome analysis are currently used in plant epigenomics, including genome-scale bisulfite sequencing. However, this most advanced, direct approach is feasible only for model species with small genomes - for other species, including cereal crops, indirect techniques of DNA methylome analysis are widely used. One of these is a simple, but reliable Methylation Sensitive Amplified Polymorphism (MSAP) method, that is particularly useful in studies of methylation variation. In combination with next-generation sequencing this method (called MSAP-Seq) can be successfully applied in the global analysis of DNA methylation as an alternative to whole genome bisulfite sequencing (WGBS). MSEQER is a program, which was designed for automatic analysis of data generated by MSAP-Seq method. To maximize ease of use, the program is available as a web application.

Sample data

Basic analysis steps

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